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S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-li...

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Detalles Bibliográficos
Autores principales: Peakman, Luke J., Szczelkun, Mark D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709564/
https://www.ncbi.nlm.nih.gov/pubmed/19401438
http://dx.doi.org/10.1093/nar/gkp267
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author Peakman, Luke J.
Szczelkun, Mark D.
author_facet Peakman, Luke J.
Szczelkun, Mark D.
author_sort Peakman, Luke J.
collection PubMed
description In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-like processing of the DNA is also observed that can even result in digestion of non-specific DNA in trans. We suggest a model for how DNA communication events initiating from non-specific sites, and in particular free DNA ends, could produce the observed cleavage patterns.
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spelling pubmed-27095642009-07-14 S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI Peakman, Luke J. Szczelkun, Mark D. Nucleic Acids Res Nucleic Acids Enzymes In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-like processing of the DNA is also observed that can even result in digestion of non-specific DNA in trans. We suggest a model for how DNA communication events initiating from non-specific sites, and in particular free DNA ends, could produce the observed cleavage patterns. Oxford University Press 2009-07 2009-04-28 /pmc/articles/PMC2709564/ /pubmed/19401438 http://dx.doi.org/10.1093/nar/gkp267 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nucleic Acids Enzymes
Peakman, Luke J.
Szczelkun, Mark D.
S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title_full S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title_fullStr S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title_full_unstemmed S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title_short S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
title_sort s-adenosyl homocysteine and dna ends stimulate promiscuous nuclease activities in the type iii restriction endonuclease ecopi
topic Nucleic Acids Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709564/
https://www.ncbi.nlm.nih.gov/pubmed/19401438
http://dx.doi.org/10.1093/nar/gkp267
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