Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect

BACKGROUND: CD26 is a type II, cell surface glycoprotein known as dipeptidyl peptidase (DPP) IV. Previous studies have revealed CD26 expression in T cell leukemia/lymphoma and malignant mesothelioma, and an inhibitory effect of anti-CD26 monoclonal antibody (mAb) against the growth of CD26+ cancer c...

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Autores principales: Yamada, Kohji, Hayashi, Mutsumi, Du, Wenlin, Ohnuma, Kei, Sakamoto, Michiie, Morimoto, Chikao, Yamada, Taketo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709605/
https://www.ncbi.nlm.nih.gov/pubmed/19555512
http://dx.doi.org/10.1186/1475-2867-9-17
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author Yamada, Kohji
Hayashi, Mutsumi
Du, Wenlin
Ohnuma, Kei
Sakamoto, Michiie
Morimoto, Chikao
Yamada, Taketo
author_facet Yamada, Kohji
Hayashi, Mutsumi
Du, Wenlin
Ohnuma, Kei
Sakamoto, Michiie
Morimoto, Chikao
Yamada, Taketo
author_sort Yamada, Kohji
collection PubMed
description BACKGROUND: CD26 is a type II, cell surface glycoprotein known as dipeptidyl peptidase (DPP) IV. Previous studies have revealed CD26 expression in T cell leukemia/lymphoma and malignant mesothelioma, and an inhibitory effect of anti-CD26 monoclonal antibody (mAb) against the growth of CD26+ cancer cells in vitro and in vivo. The function of CD26 in tumor development is unknown and the machinery with which the CD26 mAb induces its anti-tumor effect remains uncharacterized. RESULTS: The localization of CD26 in the nucleus of T cell leukemia/lymphoma cells and mesothelioma cells was shown by biochemical and immuno-electron microscopic analysis. The DPPIV enzyme activity was revealed in the nuclear fraction of T cell leukemia/lymphoma cells. These expressions of intra-nuclear CD26 were augmented by treatment with the CD26 mAb, 1F7, with anti-tumor effect against the CD26+ T cell leukemia/lymphoma cells. In contrast, the CD26 mAb, 5F8, without anti-tumor effect, did not augment CD26 expressions in the nucleus. Biotin-labeled, cell surface CD26 translocated into the nucleus constantly, and this translocation was enhanced with 1F7 treatment but not with 5F8. CONCLUSION: These results indicate that the intra-nuclear CD26 which moves from plasma membrane may play certain roles in cell growth of human cancer cells.
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spelling pubmed-27096052009-07-14 Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect Yamada, Kohji Hayashi, Mutsumi Du, Wenlin Ohnuma, Kei Sakamoto, Michiie Morimoto, Chikao Yamada, Taketo Cancer Cell Int Primary Research BACKGROUND: CD26 is a type II, cell surface glycoprotein known as dipeptidyl peptidase (DPP) IV. Previous studies have revealed CD26 expression in T cell leukemia/lymphoma and malignant mesothelioma, and an inhibitory effect of anti-CD26 monoclonal antibody (mAb) against the growth of CD26+ cancer cells in vitro and in vivo. The function of CD26 in tumor development is unknown and the machinery with which the CD26 mAb induces its anti-tumor effect remains uncharacterized. RESULTS: The localization of CD26 in the nucleus of T cell leukemia/lymphoma cells and mesothelioma cells was shown by biochemical and immuno-electron microscopic analysis. The DPPIV enzyme activity was revealed in the nuclear fraction of T cell leukemia/lymphoma cells. These expressions of intra-nuclear CD26 were augmented by treatment with the CD26 mAb, 1F7, with anti-tumor effect against the CD26+ T cell leukemia/lymphoma cells. In contrast, the CD26 mAb, 5F8, without anti-tumor effect, did not augment CD26 expressions in the nucleus. Biotin-labeled, cell surface CD26 translocated into the nucleus constantly, and this translocation was enhanced with 1F7 treatment but not with 5F8. CONCLUSION: These results indicate that the intra-nuclear CD26 which moves from plasma membrane may play certain roles in cell growth of human cancer cells. BioMed Central 2009-06-26 /pmc/articles/PMC2709605/ /pubmed/19555512 http://dx.doi.org/10.1186/1475-2867-9-17 Text en Copyright © 2009 Yamada et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primary Research
Yamada, Kohji
Hayashi, Mutsumi
Du, Wenlin
Ohnuma, Kei
Sakamoto, Michiie
Morimoto, Chikao
Yamada, Taketo
Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title_full Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title_fullStr Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title_full_unstemmed Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title_short Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
title_sort localization of cd26/dppiv in nucleus and its nuclear translocation enhanced by anti-cd26 monoclonal antibody with anti-tumor effect
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709605/
https://www.ncbi.nlm.nih.gov/pubmed/19555512
http://dx.doi.org/10.1186/1475-2867-9-17
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