Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

BACKGROUND: In protein engineering, site-directed mutagenesis methods are used to generate DNA sequences with mutated codons, insertions or deletions. In a widely used method, mutations are generated by PCR using a pair of oligonucleotide primers designed with mismatching nucleotides at the center o...

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Detalles Bibliográficos
Autores principales: Edelheit, Oded, Hanukoglu, Aaron, Hanukoglu, Israel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711942/
https://www.ncbi.nlm.nih.gov/pubmed/19566935
http://dx.doi.org/10.1186/1472-6750-9-61

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711942/
https://www.ncbi.nlm.nih.gov/pubmed/19566935
http://dx.doi.org/10.1186/1472-6750-9-61

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