Selection and evaluation of reference genes for improved interrogation of microbial transcriptomes: case study with the extremophile Acidithiobacillus ferrooxidans

BACKGROUND: Normalization is a prerequisite for accurate real time PCR (qPCR) expression analysis and for the validation of microarray profiling data in microbial systems. The choice and use of reference genes that are stably expressed across samples, experimental conditions and designs is a key con...

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Detalles Bibliográficos
Autores principales: Nieto, Pamela A, Covarrubias, Paulo C, Jedlicki, Eugenia, Holmes, David S, Quatrini, Raquel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713239/
https://www.ncbi.nlm.nih.gov/pubmed/19555508
http://dx.doi.org/10.1186/1471-2199-10-63

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713239/
https://www.ncbi.nlm.nih.gov/pubmed/19555508
http://dx.doi.org/10.1186/1471-2199-10-63

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