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Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular poly...

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Detalles Bibliográficos
Autores principales: Quan, Jiayuan, Tian, Jingdong
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713398/
https://www.ncbi.nlm.nih.gov/pubmed/19649325
http://dx.doi.org/10.1371/journal.pone.0006441