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Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular poly...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713398/ https://www.ncbi.nlm.nih.gov/pubmed/19649325 http://dx.doi.org/10.1371/journal.pone.0006441 |