A thermodynamic approach to PCR primer design

We developed a primer design method, Pythia, in which state of the art DNA binding affinity computations are directly integrated into the primer design process. We use chemical reaction equilibrium analysis to integrate multiple binding energy calculations into a conservative measure of polymerase c...

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Detalles Bibliográficos
Autores principales: Mann, Tobias, Humbert, Richard, Dorschner, Michael, Stamatoyannopoulos, John, Noble, William Stafford
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715258/
https://www.ncbi.nlm.nih.gov/pubmed/19528077
http://dx.doi.org/10.1093/nar/gkp443

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715258/
https://www.ncbi.nlm.nih.gov/pubmed/19528077
http://dx.doi.org/10.1093/nar/gkp443

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