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p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p′-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p′-DDE exposure causes male...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717566/ https://www.ncbi.nlm.nih.gov/pubmed/19644561 http://dx.doi.org/10.1155/2009/181282 |
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author | Shi, Yuqin Song, Yang Wang, Yinan Liang, Xianmin Hu, Yafei Guan, Xia Cheng, Jin Yang, Kedi |
author_facet | Shi, Yuqin Song, Yang Wang, Yinan Liang, Xianmin Hu, Yafei Guan, Xia Cheng, Jin Yang, Kedi |
author_sort | Shi, Yuqin |
collection | PubMed |
description | One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p′-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p′-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p′-DDE-induced toxicity in male reproductive system. The results indicated that p,p′-DDE exposure at over 30 μM showed the induction of apoptotic cell death. p,p′-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p′-DDE treatment in these cells. The activation of NF-κB was enhanced with the increase of p,p′-DDE dose. Taken together, these results suggested that exposure to p,p′-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway. |
format | Text |
id | pubmed-2717566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-27175662009-07-30 p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway Shi, Yuqin Song, Yang Wang, Yinan Liang, Xianmin Hu, Yafei Guan, Xia Cheng, Jin Yang, Kedi J Biomed Biotechnol Research Article One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p′-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p′-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p′-DDE-induced toxicity in male reproductive system. The results indicated that p,p′-DDE exposure at over 30 μM showed the induction of apoptotic cell death. p,p′-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p′-DDE treatment in these cells. The activation of NF-κB was enhanced with the increase of p,p′-DDE dose. Taken together, these results suggested that exposure to p,p′-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway. Hindawi Publishing Corporation 2009 2009-07-22 /pmc/articles/PMC2717566/ /pubmed/19644561 http://dx.doi.org/10.1155/2009/181282 Text en Copyright © 2009 Yuqin Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shi, Yuqin Song, Yang Wang, Yinan Liang, Xianmin Hu, Yafei Guan, Xia Cheng, Jin Yang, Kedi p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title | p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title_full | p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title_fullStr | p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title_full_unstemmed | p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title_short | p,p′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway |
title_sort | p,p′-dde induces apoptosis of rat sertoli cells via a fasl-dependent pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717566/ https://www.ncbi.nlm.nih.gov/pubmed/19644561 http://dx.doi.org/10.1155/2009/181282 |
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