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Application of reverse-phase HPLC to quantify oligopeptide acetylation eliminates interference from unspecific acetyl CoA hydrolysis

Protein acetylation is a common modification that plays a central role in several cellular processes. The most widely used methods to study these modifications are either based on the detection of radioactively acetylated oligopetide products or an enzyme-coupled reaction measuring conversion of the...

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Detalles Bibliográficos
Autores principales: Evjenth, Rune, Hole, Kristine, Ziegler, Mathias, Lillehaug, Johan R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722098/
https://www.ncbi.nlm.nih.gov/pubmed/19660098
http://dx.doi.org/10.1186/1753-6561-3-S6-S5