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A comparison of RNA amplification techniques at sub-nanogram input concentration

BACKGROUND: Gene expression profiling of small numbers of cells requires high-fidelity amplification of sub-nanogram amounts of RNA. Several methods for RNA amplification are available; however, there has been little consideration of the accuracy of these methods when working with very low-input qua...

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Detalles Bibliográficos
Autores principales: Lang, Julie E, Magbanua, Mark Jesus M, Scott, Janet H, Makrigiorgos, G Mike, Wang, Gang, Federman, Scot, Esserman, Laura J, Park, John W, Haqq, Christopher M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724417/
https://www.ncbi.nlm.nih.gov/pubmed/19619282
http://dx.doi.org/10.1186/1471-2164-10-326