In Vivo Analysis of the Notch Receptor S1 Cleavage

A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear an...

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Detalles Bibliográficos
Autores principales: Lake, Robert J., Grimm, Lisa M., Veraksa, Alexey, Banos, Andrew, Artavanis-Tsakonas, Spyros
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726433/
https://www.ncbi.nlm.nih.gov/pubmed/19701455
http://dx.doi.org/10.1371/journal.pone.0006728
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author Lake, Robert J.
Grimm, Lisa M.
Veraksa, Alexey
Banos, Andrew
Artavanis-Tsakonas, Spyros
author_facet Lake, Robert J.
Grimm, Lisa M.
Veraksa, Alexey
Banos, Andrew
Artavanis-Tsakonas, Spyros
author_sort Lake, Robert J.
collection PubMed
description A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
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spelling pubmed-27264332009-08-24 In Vivo Analysis of the Notch Receptor S1 Cleavage Lake, Robert J. Grimm, Lisa M. Veraksa, Alexey Banos, Andrew Artavanis-Tsakonas, Spyros PLoS One Research Article A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control. Public Library of Science 2009-08-24 /pmc/articles/PMC2726433/ /pubmed/19701455 http://dx.doi.org/10.1371/journal.pone.0006728 Text en Lake et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lake, Robert J.
Grimm, Lisa M.
Veraksa, Alexey
Banos, Andrew
Artavanis-Tsakonas, Spyros
In Vivo Analysis of the Notch Receptor S1 Cleavage
title In Vivo Analysis of the Notch Receptor S1 Cleavage
title_full In Vivo Analysis of the Notch Receptor S1 Cleavage
title_fullStr In Vivo Analysis of the Notch Receptor S1 Cleavage
title_full_unstemmed In Vivo Analysis of the Notch Receptor S1 Cleavage
title_short In Vivo Analysis of the Notch Receptor S1 Cleavage
title_sort in vivo analysis of the notch receptor s1 cleavage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726433/
https://www.ncbi.nlm.nih.gov/pubmed/19701455
http://dx.doi.org/10.1371/journal.pone.0006728
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AT banosandrew invivoanalysisofthenotchreceptors1cleavage
AT artavanistsakonasspyros invivoanalysisofthenotchreceptors1cleavage

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