A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells

Avian influenza viruses (AIV), the causative agent of avian flu or bird flu, cause widespread morbidity and mortality in poultry. The symptoms of the disease range from mild flu like symptoms to death. These viruses possess two important surface glycoproteins, namely hemagglutinin (HA) and neuramini...

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Autores principales: Rajik, Mohamed, Omar, Abdul Rahman, Ideris, Aini, Hassan, Sharifah Syed, Yusoff, Khatijah
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726580/
https://www.ncbi.nlm.nih.gov/pubmed/19680476
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author Rajik, Mohamed
Omar, Abdul Rahman
Ideris, Aini
Hassan, Sharifah Syed
Yusoff, Khatijah
author_facet Rajik, Mohamed
Omar, Abdul Rahman
Ideris, Aini
Hassan, Sharifah Syed
Yusoff, Khatijah
author_sort Rajik, Mohamed
collection PubMed
description Avian influenza viruses (AIV), the causative agent of avian flu or bird flu, cause widespread morbidity and mortality in poultry. The symptoms of the disease range from mild flu like symptoms to death. These viruses possess two important surface glycoproteins, namely hemagglutinin (HA) and neuraminidase (NA) against which neutralizing antibodies are produced. Due to the highly mutative nature of the genes which encode these proteins, the viruses often confer resistance to the current anti-viral drugs making the prevention and treatment of infection challenging. In our laboratory, we have recently identified a novel anti-viral peptide (P1) against the AIV H9N2 from a phage displayed peptide library. This peptide inhibits the replication of the virus in ovo and in vitro by its binding to the HA glycoprotein. In the current study, we demonstrate that the peptide inhibits the virus replication by preventing the attachment to the host cell but it does not have any effect on the viral fusion. The reduction in the viral nucleoprotein (NP) expression inside the host cell has also been observed during the peptide (P1) treatment. This novel peptide may have the potential to be developed as a therapeutic agent for the treatment and control of avian influenza virus H9N2 infections.
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spelling pubmed-27265802009-08-13 A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells Rajik, Mohamed Omar, Abdul Rahman Ideris, Aini Hassan, Sharifah Syed Yusoff, Khatijah Int J Biol Sci Short Research Communication Avian influenza viruses (AIV), the causative agent of avian flu or bird flu, cause widespread morbidity and mortality in poultry. The symptoms of the disease range from mild flu like symptoms to death. These viruses possess two important surface glycoproteins, namely hemagglutinin (HA) and neuraminidase (NA) against which neutralizing antibodies are produced. Due to the highly mutative nature of the genes which encode these proteins, the viruses often confer resistance to the current anti-viral drugs making the prevention and treatment of infection challenging. In our laboratory, we have recently identified a novel anti-viral peptide (P1) against the AIV H9N2 from a phage displayed peptide library. This peptide inhibits the replication of the virus in ovo and in vitro by its binding to the HA glycoprotein. In the current study, we demonstrate that the peptide inhibits the virus replication by preventing the attachment to the host cell but it does not have any effect on the viral fusion. The reduction in the viral nucleoprotein (NP) expression inside the host cell has also been observed during the peptide (P1) treatment. This novel peptide may have the potential to be developed as a therapeutic agent for the treatment and control of avian influenza virus H9N2 infections. Ivyspring International Publisher 2009-08-08 /pmc/articles/PMC2726580/ /pubmed/19680476 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Short Research Communication
Rajik, Mohamed
Omar, Abdul Rahman
Ideris, Aini
Hassan, Sharifah Syed
Yusoff, Khatijah
A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title_full A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title_fullStr A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title_full_unstemmed A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title_short A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
title_sort novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells
topic Short Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726580/
https://www.ncbi.nlm.nih.gov/pubmed/19680476
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