Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Zebrafish have long been utilized to study the cellular and molecular mechanisms of development by time-lapse imaging of the living transparent embryo. Here we describe a method to mount zebrafish embryos for long-term imaging and demonstrate how to automate the capture of time-lapse images using a...

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Detalles Bibliográficos
Autores principales: O'Brien, Georgeann S., Rieger, Sandra, Martin, Seanna M., Cavanaugh, Ann M., Portera-Cailliau, Carlos, Sagasti, Alvaro
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2009
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726581/
https://www.ncbi.nlm.nih.gov/pubmed/19229185
http://dx.doi.org/10.3791/1129

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726581/
https://www.ncbi.nlm.nih.gov/pubmed/19229185
http://dx.doi.org/10.3791/1129

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