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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
Zebrafish have long been utilized to study the cellular and molecular mechanisms of development by time-lapse imaging of the living transparent embryo. Here we describe a method to mount zebrafish embryos for long-term imaging and demonstrate how to automate the capture of time-lapse images using a...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
MyJove Corporation
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726581/ https://www.ncbi.nlm.nih.gov/pubmed/19229185 http://dx.doi.org/10.3791/1129 |