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Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa

BACKGROUND: Fermentation of dietary fiber in the colon results in the production of short chain fatty acids (mainly propionate, butyrate and acetate). Butyrate modulates a wide range of processes, but its mechanism of action is mostly unknown. This study aimed to determine the effects of butyrate on...

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Autores principales: Vanhoutvin, Steven A. L. W., Troost, Freddy J., Hamer, Henrike M., Lindsey, Patrick J., Koek, Ger H., Jonkers, Daisy M. A. E., Kodde, Andrea, Venema, Koen, Brummer, Robert J. M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727000/
https://www.ncbi.nlm.nih.gov/pubmed/19707587
http://dx.doi.org/10.1371/journal.pone.0006759
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author Vanhoutvin, Steven A. L. W.
Troost, Freddy J.
Hamer, Henrike M.
Lindsey, Patrick J.
Koek, Ger H.
Jonkers, Daisy M. A. E.
Kodde, Andrea
Venema, Koen
Brummer, Robert J. M.
author_facet Vanhoutvin, Steven A. L. W.
Troost, Freddy J.
Hamer, Henrike M.
Lindsey, Patrick J.
Koek, Ger H.
Jonkers, Daisy M. A. E.
Kodde, Andrea
Venema, Koen
Brummer, Robert J. M.
author_sort Vanhoutvin, Steven A. L. W.
collection PubMed
description BACKGROUND: Fermentation of dietary fiber in the colon results in the production of short chain fatty acids (mainly propionate, butyrate and acetate). Butyrate modulates a wide range of processes, but its mechanism of action is mostly unknown. This study aimed to determine the effects of butyrate on the transcriptional regulation of human colonic mucosa in vivo. METHODOLOGY/PRINCIPAL FINDINGS: Five hundred genes were found to be differentially expressed after a two week daily butyrate administration with enemas. Pathway analysis showed that the butyrate intervention mainly resulted in an increased transcriptional regulation of the pathways representing fatty acid oxidation, electron transport chain and oxidative stress. In addition, several genes associated with epithelial integrity and apoptosis, were found to be differentially expressed after the butyrate intervention. CONCLUSIONS/SIGNIFICANCE: Colonic administration of butyrate in concentrations that can be achieved by consumption of a high-fiber diet enhances the maintenance of colonic homeostasis in healthy subjects, by regulating fatty acid metabolism, electron transport and oxidative stress pathways on the transcriptional level and provide for the first time, detailed molecular insight in the transcriptional response of gut mucosa to butyrate.
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spelling pubmed-27270002009-08-25 Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa Vanhoutvin, Steven A. L. W. Troost, Freddy J. Hamer, Henrike M. Lindsey, Patrick J. Koek, Ger H. Jonkers, Daisy M. A. E. Kodde, Andrea Venema, Koen Brummer, Robert J. M. PLoS One Research Article BACKGROUND: Fermentation of dietary fiber in the colon results in the production of short chain fatty acids (mainly propionate, butyrate and acetate). Butyrate modulates a wide range of processes, but its mechanism of action is mostly unknown. This study aimed to determine the effects of butyrate on the transcriptional regulation of human colonic mucosa in vivo. METHODOLOGY/PRINCIPAL FINDINGS: Five hundred genes were found to be differentially expressed after a two week daily butyrate administration with enemas. Pathway analysis showed that the butyrate intervention mainly resulted in an increased transcriptional regulation of the pathways representing fatty acid oxidation, electron transport chain and oxidative stress. In addition, several genes associated with epithelial integrity and apoptosis, were found to be differentially expressed after the butyrate intervention. CONCLUSIONS/SIGNIFICANCE: Colonic administration of butyrate in concentrations that can be achieved by consumption of a high-fiber diet enhances the maintenance of colonic homeostasis in healthy subjects, by regulating fatty acid metabolism, electron transport and oxidative stress pathways on the transcriptional level and provide for the first time, detailed molecular insight in the transcriptional response of gut mucosa to butyrate. Public Library of Science 2009-08-25 /pmc/articles/PMC2727000/ /pubmed/19707587 http://dx.doi.org/10.1371/journal.pone.0006759 Text en Vanhoutvin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vanhoutvin, Steven A. L. W.
Troost, Freddy J.
Hamer, Henrike M.
Lindsey, Patrick J.
Koek, Ger H.
Jonkers, Daisy M. A. E.
Kodde, Andrea
Venema, Koen
Brummer, Robert J. M.
Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title_full Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title_fullStr Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title_full_unstemmed Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title_short Butyrate-Induced Transcriptional Changes in Human Colonic Mucosa
title_sort butyrate-induced transcriptional changes in human colonic mucosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727000/
https://www.ncbi.nlm.nih.gov/pubmed/19707587
http://dx.doi.org/10.1371/journal.pone.0006759
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