Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification

The study of functional RNAs of various sizes and structures requires efficient methods for their synthesis and purification. Here, 23 group I intron variants ranging in length from 246 to 341 nucleotides—some containing exons—were subjected to a native purification technique previously applied only...

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Detalles Bibliográficos
Autores principales: Vicens, Quentin, Gooding, Anne R., Duarte, Luis F., Batey, Robert T.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729099/
https://www.ncbi.nlm.nih.gov/pubmed/19710925
http://dx.doi.org/10.1371/journal.pone.0006740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729099/
https://www.ncbi.nlm.nih.gov/pubmed/19710925
http://dx.doi.org/10.1371/journal.pone.0006740

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