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Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification
The study of functional RNAs of various sizes and structures requires efficient methods for their synthesis and purification. Here, 23 group I intron variants ranging in length from 246 to 341 nucleotides—some containing exons—were subjected to a native purification technique previously applied only...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729099/ https://www.ncbi.nlm.nih.gov/pubmed/19710925 http://dx.doi.org/10.1371/journal.pone.0006740 |