Cargando…
Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
The preparation of a gapped pUC18 derivative, containing the lacZα reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZα gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the...
| Autores principales: | , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731893/ https://www.ncbi.nlm.nih.gov/pubmed/19515939 http://dx.doi.org/10.1093/nar/gkp494 |