Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation

A total of 814 isolates of the foodborne pathogen Campylobacter jejuni were characterized by multilocus sequence typing (MLST) and analysis of the variation of two cell-surface components: the heat-stable (HS) serotyping antigen and the flagella protein FlaA short variable region (SVR). We identifie...

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Autores principales: Dingle, Kate E., Colles, Frances M., Ure, Roisin, Wagenaar, Jaap A., Duim, Birgitta, Bolton, Frederick J., Fox, Andrew J., Wareing, David R.A., Maiden, Martin C.J.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732546/
https://www.ncbi.nlm.nih.gov/pubmed/12194772
http://dx.doi.org/10.3201/eid0809.02-0122
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author Dingle, Kate E.
Colles, Frances M.
Ure, Roisin
Wagenaar, Jaap A.
Duim, Birgitta
Bolton, Frederick J.
Fox, Andrew J.
Wareing, David R.A.
Maiden, Martin C.J.
author_facet Dingle, Kate E.
Colles, Frances M.
Ure, Roisin
Wagenaar, Jaap A.
Duim, Birgitta
Bolton, Frederick J.
Fox, Andrew J.
Wareing, David R.A.
Maiden, Martin C.J.
author_sort Dingle, Kate E.
collection PubMed
description A total of 814 isolates of the foodborne pathogen Campylobacter jejuni were characterized by multilocus sequence typing (MLST) and analysis of the variation of two cell-surface components: the heat-stable (HS) serotyping antigen and the flagella protein FlaA short variable region (SVR). We identified 379 combinations of the MLST loci (sequence types) and 215 combinations of the cell-surface components among these isolates, which had been obtained from human disease, animals, food, and the environment. Despite this diversity, 748 (92%) of the isolates belonged to one of 17 clonal complexes, 6 of which contained many (318, 63%) of the human disease isolates. Several clonal complexes exhibited associations with isolation source or particular cell-surface components; however, the latter were poorly predictive of clonal complex. These data demonstrate that the clonal complex, as defined by MLST, is an epidemiologically relevant unit for both long and short-term investigations of C. jejuni epidemiology.
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spelling pubmed-27325462009-09-16 Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation Dingle, Kate E. Colles, Frances M. Ure, Roisin Wagenaar, Jaap A. Duim, Birgitta Bolton, Frederick J. Fox, Andrew J. Wareing, David R.A. Maiden, Martin C.J. Emerg Infect Dis Research A total of 814 isolates of the foodborne pathogen Campylobacter jejuni were characterized by multilocus sequence typing (MLST) and analysis of the variation of two cell-surface components: the heat-stable (HS) serotyping antigen and the flagella protein FlaA short variable region (SVR). We identified 379 combinations of the MLST loci (sequence types) and 215 combinations of the cell-surface components among these isolates, which had been obtained from human disease, animals, food, and the environment. Despite this diversity, 748 (92%) of the isolates belonged to one of 17 clonal complexes, 6 of which contained many (318, 63%) of the human disease isolates. Several clonal complexes exhibited associations with isolation source or particular cell-surface components; however, the latter were poorly predictive of clonal complex. These data demonstrate that the clonal complex, as defined by MLST, is an epidemiologically relevant unit for both long and short-term investigations of C. jejuni epidemiology. Centers for Disease Control and Prevention 2002-09 /pmc/articles/PMC2732546/ /pubmed/12194772 http://dx.doi.org/10.3201/eid0809.02-0122 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Dingle, Kate E.
Colles, Frances M.
Ure, Roisin
Wagenaar, Jaap A.
Duim, Birgitta
Bolton, Frederick J.
Fox, Andrew J.
Wareing, David R.A.
Maiden, Martin C.J.
Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title_full Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title_fullStr Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title_full_unstemmed Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title_short Molecular Characterization of Campylobacter jejuni Clones: A Basis for Epidemiologic Investigation
title_sort molecular characterization of campylobacter jejuni clones: a basis for epidemiologic investigation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732546/
https://www.ncbi.nlm.nih.gov/pubmed/12194772
http://dx.doi.org/10.3201/eid0809.02-0122
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