RNAi induced in mammalian and Drosophila cells via transfection of dimers and trimers of small interfering RNA

Twenty one base pair long small interfering RNAs (siRNAs) are widely in use in mammalian RNAi experiments. The present study assesses the capability of 43 and 63bp dsRNAs with two 2nt long 3′-overhangs to induce RNAi in mammalian and Drosophila cells. Human Dicer was found to cleave these dsRNAs fro...

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Detalles Bibliográficos
Autores principales: Ui-Tei, Kumiko, Zenno, Shuhei, Juni, Aya, Saigo, Kaoru
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2005
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737202/
https://www.ncbi.nlm.nih.gov/pubmed/19771208

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737202/
https://www.ncbi.nlm.nih.gov/pubmed/19771208

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