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A novel expression system for artificial miRNAs containing no endogenous miRNA precursor sequences

Recently, artificial microRNA (miRNA)-mediated RNA interference (RNAi) systems have been developed as useful tools to study gene functions. We report an artificial miRNA precursor motif (AMPM) containing several restriction sites in the loop and in the extended stem structures, and generated a vecto...

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Detalles Bibliográficos
Autores principales: Shibata, Atsushi, Iwaki, Akiko, Fukumaki, Yasuyuki
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737215/
https://www.ncbi.nlm.nih.gov/pubmed/19771221