3′-Poly(A) tail enhances siRNA activity against exogenous reporter genes in MCF-7 cells

RNA interference (RNAi) is a powerful tool in the study of gene function. We added poly(A) tails to the 3′ ends of siRNA antisense strands by in vitro transcription, and investigated the silencing effects of poly (A)-tailed siRNAs on enhanced green fluorescence protein (EGFP) and red fluorescence pr...

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Detalles Bibliográficos
Autores principales: Li, Jie, Yang, Guang, Li, Shaohua, Cao, Guojun, Zhao, Qiang, Liu, Xuemei, Fan, Ming, Shen, Beifen, Shao, Ningsheng
Formato: Texto
Lenguaje:English
Publicado: Library Publishing Media 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737218/
https://www.ncbi.nlm.nih.gov/pubmed/19771226

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737218/
https://www.ncbi.nlm.nih.gov/pubmed/19771226

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