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3′-Poly(A) tail enhances siRNA activity against exogenous reporter genes in MCF-7 cells
RNA interference (RNAi) is a powerful tool in the study of gene function. We added poly(A) tails to the 3′ ends of siRNA antisense strands by in vitro transcription, and investigated the silencing effects of poly (A)-tailed siRNAs on enhanced green fluorescence protein (EGFP) and red fluorescence pr...
Autores principales: | Li, Jie, Yang, Guang, Li, Shaohua, Cao, Guojun, Zhao, Qiang, Liu, Xuemei, Fan, Ming, Shen, Beifen, Shao, Ningsheng |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Library Publishing Media
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737218/ https://www.ncbi.nlm.nih.gov/pubmed/19771226 |
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