Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

BACKGROUND: PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most pernicious source of contamination is microbi...

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Detalles Bibliográficos
Autores principales: Spangler, Rudolph, Goddard, Noel L., Thaler, David S.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737620/
https://www.ncbi.nlm.nih.gov/pubmed/19753123
http://dx.doi.org/10.1371/journal.pone.0007010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737620/
https://www.ncbi.nlm.nih.gov/pubmed/19753123
http://dx.doi.org/10.1371/journal.pone.0007010

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