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Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria
BACKGROUND: PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most pernicious source of contamination is microbi...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
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Public Library of Science
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737620/ https://www.ncbi.nlm.nih.gov/pubmed/19753123 http://dx.doi.org/10.1371/journal.pone.0007010 |