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Mapping the structure and conformational movements of proteins with transition metal ion FRET
Visualizing conformational dynamics in proteins has been difficult, and the atomic-scale motions responsible for the behavior of most allosteric proteins are unknown. Here, we report that FRET between a small fluorescent dye and a nickel ion bound to a di-histidine motif can be used to monitor small...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738593/ https://www.ncbi.nlm.nih.gov/pubmed/19525958 http://dx.doi.org/10.1038/nmeth.1341 |