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Mapping the structure and conformational movements of proteins with transition metal ion FRET

Visualizing conformational dynamics in proteins has been difficult, and the atomic-scale motions responsible for the behavior of most allosteric proteins are unknown. Here, we report that FRET between a small fluorescent dye and a nickel ion bound to a di-histidine motif can be used to monitor small...

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Detalles Bibliográficos
Autores principales: Taraska, Justin W., Puljung, Michael C., Olivier, Nelson B., Flynn, Galen E., Zagotta, William N.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738593/
https://www.ncbi.nlm.nih.gov/pubmed/19525958
http://dx.doi.org/10.1038/nmeth.1341