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Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration
BACKGROUND: CCR7-mediated signalling is important for dendritic cell maturation and homing to the lymph nodes. We have previously demonstrated that Jak3 participates in the signalling pathway of CCR7 in T lymphocytes. METHODOLOGY AND PRINCIPAL FINDINGS: Here, we used Jak3(−/−) mice to analyze the ro...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738966/ https://www.ncbi.nlm.nih.gov/pubmed/19759904 http://dx.doi.org/10.1371/journal.pone.0007066 |
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author | Rivas-Caicedo, Ana Soldevila, Gloria Fortoul, Teresa I. Castell-Rodríguez, Andrés Flores-Romo, Leopoldo García-Zepeda, Eduardo A. |
author_facet | Rivas-Caicedo, Ana Soldevila, Gloria Fortoul, Teresa I. Castell-Rodríguez, Andrés Flores-Romo, Leopoldo García-Zepeda, Eduardo A. |
author_sort | Rivas-Caicedo, Ana |
collection | PubMed |
description | BACKGROUND: CCR7-mediated signalling is important for dendritic cell maturation and homing to the lymph nodes. We have previously demonstrated that Jak3 participates in the signalling pathway of CCR7 in T lymphocytes. METHODOLOGY AND PRINCIPAL FINDINGS: Here, we used Jak3(−/−) mice to analyze the role of Jak3 in CCR7-mediated dendritic cells migration and function. First, we found no differences in the generation of DCs from Jak3(−/−) bone marrow progenitors, when compared to wild type cells. However, phenotypic analysis of the bone marrow derived DCs obtained from Jak3(−/−) mice showed reduced expression of co-stimulatory molecules compared to wild type (Jak3(+/+)). In addition, when we analyzed the migration of Jak3(−/−) and Jak3(+/+) mature DCs in response to CCL19 and CCL21 chemokines, we found that the absence of Jak3 results in impaired chemotactic responses both in vitro and in vivo. Moreover, lymphocyte proliferation and contact hypersensitivity experiments showed that DC-mediated T lymphocyte activation is reduced in the absence of Jak3. CONCLUSION/SIGNIFICANCE: Altogether, our data provide strong evidence that Jak3 is important for DC maturation, migration and function, through a CCR7-mediated signalling pathway. |
format | Text |
id | pubmed-2738966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27389662009-09-17 Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration Rivas-Caicedo, Ana Soldevila, Gloria Fortoul, Teresa I. Castell-Rodríguez, Andrés Flores-Romo, Leopoldo García-Zepeda, Eduardo A. PLoS One Research Article BACKGROUND: CCR7-mediated signalling is important for dendritic cell maturation and homing to the lymph nodes. We have previously demonstrated that Jak3 participates in the signalling pathway of CCR7 in T lymphocytes. METHODOLOGY AND PRINCIPAL FINDINGS: Here, we used Jak3(−/−) mice to analyze the role of Jak3 in CCR7-mediated dendritic cells migration and function. First, we found no differences in the generation of DCs from Jak3(−/−) bone marrow progenitors, when compared to wild type cells. However, phenotypic analysis of the bone marrow derived DCs obtained from Jak3(−/−) mice showed reduced expression of co-stimulatory molecules compared to wild type (Jak3(+/+)). In addition, when we analyzed the migration of Jak3(−/−) and Jak3(+/+) mature DCs in response to CCL19 and CCL21 chemokines, we found that the absence of Jak3 results in impaired chemotactic responses both in vitro and in vivo. Moreover, lymphocyte proliferation and contact hypersensitivity experiments showed that DC-mediated T lymphocyte activation is reduced in the absence of Jak3. CONCLUSION/SIGNIFICANCE: Altogether, our data provide strong evidence that Jak3 is important for DC maturation, migration and function, through a CCR7-mediated signalling pathway. Public Library of Science 2009-09-17 /pmc/articles/PMC2738966/ /pubmed/19759904 http://dx.doi.org/10.1371/journal.pone.0007066 Text en Rivas-Caicedo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rivas-Caicedo, Ana Soldevila, Gloria Fortoul, Teresa I. Castell-Rodríguez, Andrés Flores-Romo, Leopoldo García-Zepeda, Eduardo A. Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title | Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title_full | Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title_fullStr | Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title_full_unstemmed | Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title_short | Jak3 Is Involved in Dendritic Cell Maturation and CCR7-Dependent Migration |
title_sort | jak3 is involved in dendritic cell maturation and ccr7-dependent migration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2738966/ https://www.ncbi.nlm.nih.gov/pubmed/19759904 http://dx.doi.org/10.1371/journal.pone.0007066 |
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