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The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro
BACKGROUND: Despite of the recent success of EGFR inhibitory agents, the primary drug-resistant becomes a major challenge for EGFR inhibitor therapies. PTEN gene is an important positive regulatory factor for response to EGFR inhibitor therapy. Low-expression of PTEN is clearly one of the important...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741431/ https://www.ncbi.nlm.nih.gov/pubmed/19723324 http://dx.doi.org/10.1186/1756-9966-28-123 |
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author | Zhuang, Hong-Qing Wang, Jun Yuan, Zhi-Yong Zhao, Lu-Jun Wang, Ping Wang, Chang-Li |
author_facet | Zhuang, Hong-Qing Wang, Jun Yuan, Zhi-Yong Zhao, Lu-Jun Wang, Ping Wang, Chang-Li |
author_sort | Zhuang, Hong-Qing |
collection | PubMed |
description | BACKGROUND: Despite of the recent success of EGFR inhibitory agents, the primary drug-resistant becomes a major challenge for EGFR inhibitor therapies. PTEN gene is an important positive regulatory factor for response to EGFR inhibitor therapy. Low-expression of PTEN is clearly one of the important reasons why tumor cells resisted to tyrosine kinase inhibitors. METHODS: To investigate the drug-resistance reversal to gefitinb and the mechanism in PTEN low expression cells which radiated with X-rays in vitro, We demonstrated that H-157 lung cancer cells (low-expression of PTEN but phospho-EGFR overexpressed tumor cells) exposed to X-rays. The PTEN expressions and radiosensitizing effects of tyrosine kinase inhibitor before and after irradiation were observed. The cell-survival rates were evaluated by colony-forming assays. The cell apoptosis was investigated using FCM. The expressions of phospho-EGFR and PTEN were determined by Western blot analysis. RESULTS: The results showed that the PTEN expressions were significantly enhanced by X-rays. Moreover, the cell growth curve and survival curve were down-regulated in the gefitinib-treated groups after irradiation. Meanwhile, the radiation-induced apoptosis of tumor cells was increased by inhibition of the EGFR through up-regulation of PTEN. CONCLUSION: These results suggested that PTEN gene is an important regulator on TKI inhibition, and the resistance to tyrosine kinase inhibitors might be reversed by irradiation in PTEN low expression cancer cells. |
format | Text |
id | pubmed-2741431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27414312009-09-11 The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro Zhuang, Hong-Qing Wang, Jun Yuan, Zhi-Yong Zhao, Lu-Jun Wang, Ping Wang, Chang-Li J Exp Clin Cancer Res Research BACKGROUND: Despite of the recent success of EGFR inhibitory agents, the primary drug-resistant becomes a major challenge for EGFR inhibitor therapies. PTEN gene is an important positive regulatory factor for response to EGFR inhibitor therapy. Low-expression of PTEN is clearly one of the important reasons why tumor cells resisted to tyrosine kinase inhibitors. METHODS: To investigate the drug-resistance reversal to gefitinb and the mechanism in PTEN low expression cells which radiated with X-rays in vitro, We demonstrated that H-157 lung cancer cells (low-expression of PTEN but phospho-EGFR overexpressed tumor cells) exposed to X-rays. The PTEN expressions and radiosensitizing effects of tyrosine kinase inhibitor before and after irradiation were observed. The cell-survival rates were evaluated by colony-forming assays. The cell apoptosis was investigated using FCM. The expressions of phospho-EGFR and PTEN were determined by Western blot analysis. RESULTS: The results showed that the PTEN expressions were significantly enhanced by X-rays. Moreover, the cell growth curve and survival curve were down-regulated in the gefitinib-treated groups after irradiation. Meanwhile, the radiation-induced apoptosis of tumor cells was increased by inhibition of the EGFR through up-regulation of PTEN. CONCLUSION: These results suggested that PTEN gene is an important regulator on TKI inhibition, and the resistance to tyrosine kinase inhibitors might be reversed by irradiation in PTEN low expression cancer cells. BioMed Central 2009-09-01 /pmc/articles/PMC2741431/ /pubmed/19723324 http://dx.doi.org/10.1186/1756-9966-28-123 Text en Copyright © 2009 Zhuang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zhuang, Hong-Qing Wang, Jun Yuan, Zhi-Yong Zhao, Lu-Jun Wang, Ping Wang, Chang-Li The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title | The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title_full | The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title_fullStr | The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title_full_unstemmed | The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title_short | The drug-resistance to gefitinib in PTEN low expression cancer cells is reversed by irradiation in vitro |
title_sort | drug-resistance to gefitinib in pten low expression cancer cells is reversed by irradiation in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741431/ https://www.ncbi.nlm.nih.gov/pubmed/19723324 http://dx.doi.org/10.1186/1756-9966-28-123 |
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