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Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival
BACKGROUND: DNA aneuploidy reflects gross genomic changes. It can be measured by flow cytometry (FCM-DNA) or image cytometry (ICM-DNA). In gastric cancer, the prevalence of DNA aneuploidy has been reported to range from 27 to 100%, with conflicting associations with clinicopathological variables. Th...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743350/ https://www.ncbi.nlm.nih.gov/pubmed/19738619 http://dx.doi.org/10.1038/sj.bjc.6605266 |
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author | Belien, J A M Buffart, T E Gill, A J Broeckaert, M A M Quirke, P Meijer, G A Grabsch, H I |
author_facet | Belien, J A M Buffart, T E Gill, A J Broeckaert, M A M Quirke, P Meijer, G A Grabsch, H I |
author_sort | Belien, J A M |
collection | PubMed |
description | BACKGROUND: DNA aneuploidy reflects gross genomic changes. It can be measured by flow cytometry (FCM-DNA) or image cytometry (ICM-DNA). In gastric cancer, the prevalence of DNA aneuploidy has been reported to range from 27 to 100%, with conflicting associations with clinicopathological variables. The aim of our study was to compare the DNA ploidy status measured using FCM-DNA and ICM-DNA in gastric cancer and to evaluate its association with clinicopathological variables. METHODS: Cell nuclei were isolated from 221 formalin-fixed, paraffin-embedded gastric cancer samples. DNA ploidy was assessed using FCM-DNA and ICM-DNA. RESULTS: A total of 178 (80.5%) gastric cancer samples were classified as DNA aneuploid using FCM-DNA, compared with 172 (77.8%) gastric cancer samples when using ICM-DNA. Results obtained from both methods were concordant in 183 (82.8%) cases (κ=0.48). Patients with ICM-DNA diploid gastric cancer survived significantly longer than those with ICM-DNA aneuploid gastric cancer (log rank 10.1, P=0.001). For FCM-DNA data, this difference did not reach statistical significance. The multivariate Cox model showed that ICM-DNA ploidy status predicted patient survival independently of tumour-node-metastasis status. CONCLUSION: ICM-DNA ploidy status is an independent predictor of survival in gastric cancer patients and may therefore be a more clinically relevant read out of gross genomic damage than FCM-DNA. |
format | Text |
id | pubmed-2743350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-27433502010-09-15 Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival Belien, J A M Buffart, T E Gill, A J Broeckaert, M A M Quirke, P Meijer, G A Grabsch, H I Br J Cancer Genetics and Genomics BACKGROUND: DNA aneuploidy reflects gross genomic changes. It can be measured by flow cytometry (FCM-DNA) or image cytometry (ICM-DNA). In gastric cancer, the prevalence of DNA aneuploidy has been reported to range from 27 to 100%, with conflicting associations with clinicopathological variables. The aim of our study was to compare the DNA ploidy status measured using FCM-DNA and ICM-DNA in gastric cancer and to evaluate its association with clinicopathological variables. METHODS: Cell nuclei were isolated from 221 formalin-fixed, paraffin-embedded gastric cancer samples. DNA ploidy was assessed using FCM-DNA and ICM-DNA. RESULTS: A total of 178 (80.5%) gastric cancer samples were classified as DNA aneuploid using FCM-DNA, compared with 172 (77.8%) gastric cancer samples when using ICM-DNA. Results obtained from both methods were concordant in 183 (82.8%) cases (κ=0.48). Patients with ICM-DNA diploid gastric cancer survived significantly longer than those with ICM-DNA aneuploid gastric cancer (log rank 10.1, P=0.001). For FCM-DNA data, this difference did not reach statistical significance. The multivariate Cox model showed that ICM-DNA ploidy status predicted patient survival independently of tumour-node-metastasis status. CONCLUSION: ICM-DNA ploidy status is an independent predictor of survival in gastric cancer patients and may therefore be a more clinically relevant read out of gross genomic damage than FCM-DNA. Nature Publishing Group 2009-09-15 2009-09-08 /pmc/articles/PMC2743350/ /pubmed/19738619 http://dx.doi.org/10.1038/sj.bjc.6605266 Text en Copyright © 2009 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Genetics and Genomics Belien, J A M Buffart, T E Gill, A J Broeckaert, M A M Quirke, P Meijer, G A Grabsch, H I Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title | Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title_full | Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title_fullStr | Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title_full_unstemmed | Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title_short | Gross genomic damage measured by DNA image cytometry independently predicts gastric cancer patient survival |
title_sort | gross genomic damage measured by dna image cytometry independently predicts gastric cancer patient survival |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743350/ https://www.ncbi.nlm.nih.gov/pubmed/19738619 http://dx.doi.org/10.1038/sj.bjc.6605266 |
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