DNA-catalyzed sequence-specific hydrolysis of DNA

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and...

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Detalles Bibliográficos
Autores principales: Chandra, Madhavaiah, Sachdeva, Amit, Silverman, Scott K.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746877/
https://www.ncbi.nlm.nih.gov/pubmed/19684594
http://dx.doi.org/10.1038/nchembio.201
Descripción
Sumario:Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and rate enhancement of 10(8) (possibly as high as 10(14)). The new DNA catalysts require both Mn(2+) and Zn(2+), which is intriguing because many natural DNA nucleases are bimetallic protein enzymes.

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