DNA-catalyzed sequence-specific hydrolysis of DNA

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and...

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Autores principales: Chandra, Madhavaiah, Sachdeva, Amit, Silverman, Scott K.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746877/
https://www.ncbi.nlm.nih.gov/pubmed/19684594
http://dx.doi.org/10.1038/nchembio.201
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author Chandra, Madhavaiah
Sachdeva, Amit
Silverman, Scott K.
author_facet Chandra, Madhavaiah
Sachdeva, Amit
Silverman, Scott K.
author_sort Chandra, Madhavaiah
collection PubMed
description Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and rate enhancement of 10(8) (possibly as high as 10(14)). The new DNA catalysts require both Mn(2+) and Zn(2+), which is intriguing because many natural DNA nucleases are bimetallic protein enzymes.
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spelling pubmed-27468772010-04-01 DNA-catalyzed sequence-specific hydrolysis of DNA Chandra, Madhavaiah Sachdeva, Amit Silverman, Scott K. Nat Chem Biol Article Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and rate enhancement of 10(8) (possibly as high as 10(14)). The new DNA catalysts require both Mn(2+) and Zn(2+), which is intriguing because many natural DNA nucleases are bimetallic protein enzymes. 2009-08-16 2009-10 /pmc/articles/PMC2746877/ /pubmed/19684594 http://dx.doi.org/10.1038/nchembio.201 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chandra, Madhavaiah
Sachdeva, Amit
Silverman, Scott K.
DNA-catalyzed sequence-specific hydrolysis of DNA
title DNA-catalyzed sequence-specific hydrolysis of DNA
title_full DNA-catalyzed sequence-specific hydrolysis of DNA
title_fullStr DNA-catalyzed sequence-specific hydrolysis of DNA
title_full_unstemmed DNA-catalyzed sequence-specific hydrolysis of DNA
title_short DNA-catalyzed sequence-specific hydrolysis of DNA
title_sort dna-catalyzed sequence-specific hydrolysis of dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746877/
https://www.ncbi.nlm.nih.gov/pubmed/19684594
http://dx.doi.org/10.1038/nchembio.201
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