Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA

BACKGROUND: Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce la...

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Detalles Bibliográficos
Autores principales: Noad, Rob J, Stewart, Meredith, Boyce, Mark, Celma, Cristina C, Willison, Keith R, Roy, Polly
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749033/
https://www.ncbi.nlm.nih.gov/pubmed/19725957
http://dx.doi.org/10.1186/1471-2199-10-87

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749033/
https://www.ncbi.nlm.nih.gov/pubmed/19725957
http://dx.doi.org/10.1186/1471-2199-10-87

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