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Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA
BACKGROUND: Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce la...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749033/ https://www.ncbi.nlm.nih.gov/pubmed/19725957 http://dx.doi.org/10.1186/1471-2199-10-87 |