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Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)

Matrix metalloproteinases (MMPs) play a crucial role in migration of inflammatory cells into the central nervous system (CNS). Levels of MMP-9 are elevated in multiple sclerosis (MS) and predict the occurrence of new active lesions on magnetic resonance imaging (MRI). This translational study aims t...

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Autores principales: Gold, Stefan M, Sasidhar, Manda V, Morales, Laurie B, Du, Sienmi, Sicotte, Nancy L, Tiwari-Woodruff, Seema K., Voskuhl, Rhonda R
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2753699/
https://www.ncbi.nlm.nih.gov/pubmed/19668239
http://dx.doi.org/10.1038/labinvest.2009.79
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author Gold, Stefan M
Sasidhar, Manda V
Morales, Laurie B
Du, Sienmi
Sicotte, Nancy L
Tiwari-Woodruff, Seema K.
Voskuhl, Rhonda R
author_facet Gold, Stefan M
Sasidhar, Manda V
Morales, Laurie B
Du, Sienmi
Sicotte, Nancy L
Tiwari-Woodruff, Seema K.
Voskuhl, Rhonda R
author_sort Gold, Stefan M
collection PubMed
description Matrix metalloproteinases (MMPs) play a crucial role in migration of inflammatory cells into the central nervous system (CNS). Levels of MMP-9 are elevated in multiple sclerosis (MS) and predict the occurrence of new active lesions on magnetic resonance imaging (MRI). This translational study aims to determine whether in vivo treatment with the pregnancy hormone estriol affects MMP-9 levels from immune cells in patients with MS and mice with experimental autoimmune encephalomyelitis (EAE). Peripheral blood mononuclear cells (PBMCs) collected from three female MS patients treated with estriol and splenocytes from EAE mice treated with estriol, estrogen receptor (ER) α ligand, ERβ ligand or vehicle were stimulated ex vivo and analyzed for levels of MMP-9. Markers of CNS infiltration were assessed using MRI in patients and immunohistochemistry in mice. Supernatants from PBMCs obtained during estriol treatment in female MS patients showed significantly decreased MMP-9 compared to pre treatment. Decreases in MMP-9 coincided with a decrease in enhancing lesion volume on MRI. Estriol treatment of mice with EAE reduced MMP-9 in supernatants from autoantigen stimulated splenocytes, coinciding with decreased CNS infiltration by T cells and monocytes. Experiments with selective ER ligands revealed that this effect was mediated via ERα. In conclusion, estriol acting via ERα to reduce MMP-9 from immune cells is one mechanism potentially underlying the estriol-mediated reduction in enhancing lesions in MS and inflammatory lesions in EAE.
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spelling pubmed-27536992010-04-01 Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα) Gold, Stefan M Sasidhar, Manda V Morales, Laurie B Du, Sienmi Sicotte, Nancy L Tiwari-Woodruff, Seema K. Voskuhl, Rhonda R Lab Invest Article Matrix metalloproteinases (MMPs) play a crucial role in migration of inflammatory cells into the central nervous system (CNS). Levels of MMP-9 are elevated in multiple sclerosis (MS) and predict the occurrence of new active lesions on magnetic resonance imaging (MRI). This translational study aims to determine whether in vivo treatment with the pregnancy hormone estriol affects MMP-9 levels from immune cells in patients with MS and mice with experimental autoimmune encephalomyelitis (EAE). Peripheral blood mononuclear cells (PBMCs) collected from three female MS patients treated with estriol and splenocytes from EAE mice treated with estriol, estrogen receptor (ER) α ligand, ERβ ligand or vehicle were stimulated ex vivo and analyzed for levels of MMP-9. Markers of CNS infiltration were assessed using MRI in patients and immunohistochemistry in mice. Supernatants from PBMCs obtained during estriol treatment in female MS patients showed significantly decreased MMP-9 compared to pre treatment. Decreases in MMP-9 coincided with a decrease in enhancing lesion volume on MRI. Estriol treatment of mice with EAE reduced MMP-9 in supernatants from autoantigen stimulated splenocytes, coinciding with decreased CNS infiltration by T cells and monocytes. Experiments with selective ER ligands revealed that this effect was mediated via ERα. In conclusion, estriol acting via ERα to reduce MMP-9 from immune cells is one mechanism potentially underlying the estriol-mediated reduction in enhancing lesions in MS and inflammatory lesions in EAE. 2009-08-10 2009-10 /pmc/articles/PMC2753699/ /pubmed/19668239 http://dx.doi.org/10.1038/labinvest.2009.79 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gold, Stefan M
Sasidhar, Manda V
Morales, Laurie B
Du, Sienmi
Sicotte, Nancy L
Tiwari-Woodruff, Seema K.
Voskuhl, Rhonda R
Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title_full Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title_fullStr Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title_full_unstemmed Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title_short Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERα)
title_sort estrogen treatment decreases matrix metalloproteinase (mmp)-9 in autoimmune demyelinating disease through estrogen receptor alpha (erα)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2753699/
https://www.ncbi.nlm.nih.gov/pubmed/19668239
http://dx.doi.org/10.1038/labinvest.2009.79
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