Dereplication and De Novo Sequencing of Nonribosomal Peptides

Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP dereplication and sequencing. We employ multi-stage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also present an algorit...

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Detalles Bibliográficos
Autores principales: Ng, Julio, Bandeira, Nuno, Liu, Wei-Ting, Ghassemian, Majid, Simmons, Thomas L., Gerwick, William, Linington, Roger, Dorrestein, Pieter, Pevzner, Pavel
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754211/
https://www.ncbi.nlm.nih.gov/pubmed/19597502
http://dx.doi.org/10.1038/nmeth.1350
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author Ng, Julio
Bandeira, Nuno
Liu, Wei-Ting
Ghassemian, Majid
Simmons, Thomas L.
Gerwick, William
Linington, Roger
Dorrestein, Pieter
Pevzner, Pavel
author_facet Ng, Julio
Bandeira, Nuno
Liu, Wei-Ting
Ghassemian, Majid
Simmons, Thomas L.
Gerwick, William
Linington, Roger
Dorrestein, Pieter
Pevzner, Pavel
author_sort Ng, Julio
collection PubMed
description Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP dereplication and sequencing. We employ multi-stage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also present an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but non-identical NRPs, substantially reducing dereplication efforts..
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spelling pubmed-27542112010-02-01 Dereplication and De Novo Sequencing of Nonribosomal Peptides Ng, Julio Bandeira, Nuno Liu, Wei-Ting Ghassemian, Majid Simmons, Thomas L. Gerwick, William Linington, Roger Dorrestein, Pieter Pevzner, Pavel Nat Methods Article Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP dereplication and sequencing. We employ multi-stage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also present an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but non-identical NRPs, substantially reducing dereplication efforts.. 2009-07-13 2009-08 /pmc/articles/PMC2754211/ /pubmed/19597502 http://dx.doi.org/10.1038/nmeth.1350 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ng, Julio
Bandeira, Nuno
Liu, Wei-Ting
Ghassemian, Majid
Simmons, Thomas L.
Gerwick, William
Linington, Roger
Dorrestein, Pieter
Pevzner, Pavel
Dereplication and De Novo Sequencing of Nonribosomal Peptides
title Dereplication and De Novo Sequencing of Nonribosomal Peptides
title_full Dereplication and De Novo Sequencing of Nonribosomal Peptides
title_fullStr Dereplication and De Novo Sequencing of Nonribosomal Peptides
title_full_unstemmed Dereplication and De Novo Sequencing of Nonribosomal Peptides
title_short Dereplication and De Novo Sequencing of Nonribosomal Peptides
title_sort dereplication and de novo sequencing of nonribosomal peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754211/
https://www.ncbi.nlm.nih.gov/pubmed/19597502
http://dx.doi.org/10.1038/nmeth.1350
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