Cargando…

Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo

BACKGROUND: Cre recombinase is a common reagent used for the in vivo on/off switching of the expression of target genes flanked by loxP sites. In particular, recombinant TAT-Cre fusion constructs purified from bacteria have been used to promote the cell uptake of the enzyme. However, the recovery of...

Descripción completa

Detalles Bibliográficos
Autores principales: Capasso, Paola, Aliprandi, Marisa, Ossolengo, Giuseppe, Edenhofer, Frank, de Marco, Ario
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755479/
https://www.ncbi.nlm.nih.gov/pubmed/19747375
http://dx.doi.org/10.1186/1472-6750-9-80