High Yield Heterologous Expression of Wild-type and Mutant Cu(+)-ATPase (ATP7B, Wilson Disease Protein) for Functional Characterization of Catalytic Activity and Serine Residues Undergoing Copper-dependent Phosphorylation

ATP7B is a P-type ATPase required for copper homeostasis and related to Wilson disease of humans. In addition to various domains corresponding to other P-type ATPases, ATP7B includes an N terminus extension (NMBD) with six copper binding sites. We obtained high yield expression of WT and mutant ATP7...

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Detalles Bibliográficos
Autores principales: Pilankatta, Rajendra, Lewis, David, Adams, Christopher M., Inesi, Giuseppe
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2009
Materias:
Membrane Transport, Structure, Function, and Biogenesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755855/
https://www.ncbi.nlm.nih.gov/pubmed/19520855
http://dx.doi.org/10.1074/jbc.M109.023341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755855/
https://www.ncbi.nlm.nih.gov/pubmed/19520855
http://dx.doi.org/10.1074/jbc.M109.023341

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