Rapid Purification and High Sensitivity Analysis of Heparan Sulfate from Cells and Tissues: TOWARD GLYCOMICS PROFILING

Studies on glycosaminoglycans and proteoglycans (PGs) have been hampered by difficulties in isolation and analysis by traditional methods that are laborious and lack sensitivity and throughput. Here we demonstrate a simple method for rapid isolation of proteoglycans (RIP) employing phenol/guanidine/...

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Detalles Bibliográficos
Autores principales: Guimond, Scott E., Puvirajesinghe, Tania M., Skidmore, Mark A., Kalus, Ina, Dierks, Thomas, Yates, Edwin A., Turnbull, Jeremy E.
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2009
Materias:
Glycobiology and Extracellular Matrices
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757973/
https://www.ncbi.nlm.nih.gov/pubmed/19596853
http://dx.doi.org/10.1074/jbc.M109.032755

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757973/
https://www.ncbi.nlm.nih.gov/pubmed/19596853
http://dx.doi.org/10.1074/jbc.M109.032755

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