MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR

BACKGROUND: Multiple approaches for the site-directed mutagenesis (SDM) have been developed. However, only several of them are designed for simultaneous introduction of multiple nucleotide alterations, and these are time consuming. In addition, many of the existing multiple SDM methods have technica...

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Detalles Bibliográficos
Autores principales: Fushan, Alexey A, Drayna, Dennis T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759926/
https://www.ncbi.nlm.nih.gov/pubmed/19778447
http://dx.doi.org/10.1186/1472-6750-9-83

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759926/
https://www.ncbi.nlm.nih.gov/pubmed/19778447
http://dx.doi.org/10.1186/1472-6750-9-83

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