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Isolation of Cell Nuclei Using Inert Macromolecules to Mimic the Crowded Cytoplasm

Cell nuclei are commonly isolated and studied in media which include millimolar concentrations of cations, which conserve the nuclear volume by screening the negative charges on chromatin and maintaining its compaction. However, two factors question if these ionic conditions correctly reproduce the...

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Detalles Bibliográficos
Autores principales: Hancock, Ronald, Hadj-Sahraoui, Yasmina
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762040/
https://www.ncbi.nlm.nih.gov/pubmed/19851505
http://dx.doi.org/10.1371/journal.pone.0007560