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Isolation of Cell Nuclei Using Inert Macromolecules to Mimic the Crowded Cytoplasm
Cell nuclei are commonly isolated and studied in media which include millimolar concentrations of cations, which conserve the nuclear volume by screening the negative charges on chromatin and maintaining its compaction. However, two factors question if these ionic conditions correctly reproduce the...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762040/ https://www.ncbi.nlm.nih.gov/pubmed/19851505 http://dx.doi.org/10.1371/journal.pone.0007560 |