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ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor
The orphan receptor tyrosine kinase ErbB2 (HER2/Neu) transforms cells when overexpressed1, and is an important therapeutic target in human cancer2,3. Structural studies4,5 have suggested that the oncogenic (and ligand-independent) signalling properties of ErbB2 result from the absence of a key intra...
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Formato: | Texto |
Lenguaje: | English |
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2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762480/ https://www.ncbi.nlm.nih.gov/pubmed/19718021 http://dx.doi.org/10.1038/nature08297 |
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author | Alvarado, Diego Klein, Daryl E. Lemmon, Mark A. |
author_facet | Alvarado, Diego Klein, Daryl E. Lemmon, Mark A. |
author_sort | Alvarado, Diego |
collection | PubMed |
description | The orphan receptor tyrosine kinase ErbB2 (HER2/Neu) transforms cells when overexpressed1, and is an important therapeutic target in human cancer2,3. Structural studies4,5 have suggested that the oncogenic (and ligand-independent) signalling properties of ErbB2 result from the absence of a key intramolecular ‘tether’ in the extracellular region that autoinhibits other human ErbB receptors, including the epidermal growth factor (EGF) receptor6. Although ErbB2 is clearly unique among the four human ErbB receptors6,7, we show here that it is the closest structural relative of the single EGF receptor family member (dEGFR) in Drosophila melanogaster. Genetic and biochemical data show that dEGFR is tightly regulated by growth factor ligands8, yet a crystal structure shows that it too lacks the intramolecular tether seen in human EGFR, ErbB3 and ErbB4. Instead, a distinct set of autoinhibitory interdomain interactions hold unliganded dEGFR in an inactive state. All of these interactions are maintained (and even extended) in ErbB2, arguing against the suggestion that ErbB2 lacks autoinhibition. We therefore suggest that normal and pathogenic ErbB2 signalling may be regulated by ligands in the same way as dEGFR. Our findings have important implications for ErbB2 regulation in human cancer, and for developing therapeutic approaches to target novel aspects of this orphan receptor. |
format | Text |
id | pubmed-2762480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-27624802010-03-10 ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor Alvarado, Diego Klein, Daryl E. Lemmon, Mark A. Nature Article The orphan receptor tyrosine kinase ErbB2 (HER2/Neu) transforms cells when overexpressed1, and is an important therapeutic target in human cancer2,3. Structural studies4,5 have suggested that the oncogenic (and ligand-independent) signalling properties of ErbB2 result from the absence of a key intramolecular ‘tether’ in the extracellular region that autoinhibits other human ErbB receptors, including the epidermal growth factor (EGF) receptor6. Although ErbB2 is clearly unique among the four human ErbB receptors6,7, we show here that it is the closest structural relative of the single EGF receptor family member (dEGFR) in Drosophila melanogaster. Genetic and biochemical data show that dEGFR is tightly regulated by growth factor ligands8, yet a crystal structure shows that it too lacks the intramolecular tether seen in human EGFR, ErbB3 and ErbB4. Instead, a distinct set of autoinhibitory interdomain interactions hold unliganded dEGFR in an inactive state. All of these interactions are maintained (and even extended) in ErbB2, arguing against the suggestion that ErbB2 lacks autoinhibition. We therefore suggest that normal and pathogenic ErbB2 signalling may be regulated by ligands in the same way as dEGFR. Our findings have important implications for ErbB2 regulation in human cancer, and for developing therapeutic approaches to target novel aspects of this orphan receptor. 2009-08-30 2009-09-10 /pmc/articles/PMC2762480/ /pubmed/19718021 http://dx.doi.org/10.1038/nature08297 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Alvarado, Diego Klein, Daryl E. Lemmon, Mark A. ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title | ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title_full | ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title_fullStr | ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title_full_unstemmed | ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title_short | ErbB2/HER2/Neu resembles an autoinhibited invertebrate EGF receptor |
title_sort | erbb2/her2/neu resembles an autoinhibited invertebrate egf receptor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762480/ https://www.ncbi.nlm.nih.gov/pubmed/19718021 http://dx.doi.org/10.1038/nature08297 |
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