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An efficient method to assemble linear DNA templates for in vitro screening and selection systems
A method is presented to assemble a gene of interest into a linear DNA template with all the components necessary for in vitro transcription and translation in ∼90 min. Assembly is achieved using a coupled uracil excision–ligation strategy based on USER Enzyme and T4 DNA ligase, which allows the sim...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764453/ https://www.ncbi.nlm.nih.gov/pubmed/19617373 http://dx.doi.org/10.1093/nar/gkp589 |