Bacteriophage T4 endonuclease II, a promiscuous GIY-YIG nuclease, binds as a tetramer to two DNA substrates

The oligomerization state and mode of binding to DNA of the GIY-YIG endonuclease II (EndoII) from bacteriophage T4 was studied using gel filtration and electrophoretic mobility shift assays with a set of mutants previously found to have altered enzyme activity. At low enzyme/DNA ratios all mutants e...

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Detalles Bibliográficos
Autores principales: Lagerbäck, Pernilla, Andersson, Evalena, Malmberg, Christer, Carlson, Karin
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764454/
https://www.ncbi.nlm.nih.gov/pubmed/19666720
http://dx.doi.org/10.1093/nar/gkp652

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764454/
https://www.ncbi.nlm.nih.gov/pubmed/19666720
http://dx.doi.org/10.1093/nar/gkp652

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