Cargando…
AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis
Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type–specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768840/ https://www.ncbi.nlm.nih.gov/pubmed/19841138 http://dx.doi.org/10.1083/jcb.200907122 |
_version_ | 1782173519520464896 |
---|---|
author | Delevoye, Cédric Hurbain, Ilse Tenza, Danièle Sibarita, Jean-Baptiste Uzan-Gafsou, Stéphanie Ohno, Hiroshi Geerts, Willie J.C. Verkleij, Arie J. Salamero, Jean Marks, Michael S. Raposo, Graça |
author_facet | Delevoye, Cédric Hurbain, Ilse Tenza, Danièle Sibarita, Jean-Baptiste Uzan-Gafsou, Stéphanie Ohno, Hiroshi Geerts, Willie J.C. Verkleij, Arie J. Salamero, Jean Marks, Michael S. Raposo, Graça |
author_sort | Delevoye, Cédric |
collection | PubMed |
description | Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type–specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes redistributes to pericentriolar clusters, resulting in sequestration of melanosomal enzymes like Tyrp1 in vacuolar endosomes and consequent inhibition of melanin synthesis and melanosome maturation. Immunocytochemistry, live cell imaging, and electron tomography reveal AP-1– and KIF13A-dependent dynamic close appositions and continuities between peripheral endosomal tubules and melanosomes. Our results reveal that LRO protein sorting is coupled to cell type–specific positioning of endosomes that facilitate endosome–LRO contacts and are required for organelle maturation. |
format | Text |
id | pubmed-2768840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27688402010-04-19 AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis Delevoye, Cédric Hurbain, Ilse Tenza, Danièle Sibarita, Jean-Baptiste Uzan-Gafsou, Stéphanie Ohno, Hiroshi Geerts, Willie J.C. Verkleij, Arie J. Salamero, Jean Marks, Michael S. Raposo, Graça J Cell Biol Research Articles Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type–specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes redistributes to pericentriolar clusters, resulting in sequestration of melanosomal enzymes like Tyrp1 in vacuolar endosomes and consequent inhibition of melanin synthesis and melanosome maturation. Immunocytochemistry, live cell imaging, and electron tomography reveal AP-1– and KIF13A-dependent dynamic close appositions and continuities between peripheral endosomal tubules and melanosomes. Our results reveal that LRO protein sorting is coupled to cell type–specific positioning of endosomes that facilitate endosome–LRO contacts and are required for organelle maturation. The Rockefeller University Press 2009-10-19 /pmc/articles/PMC2768840/ /pubmed/19841138 http://dx.doi.org/10.1083/jcb.200907122 Text en © 2009 Delevoye et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Delevoye, Cédric Hurbain, Ilse Tenza, Danièle Sibarita, Jean-Baptiste Uzan-Gafsou, Stéphanie Ohno, Hiroshi Geerts, Willie J.C. Verkleij, Arie J. Salamero, Jean Marks, Michael S. Raposo, Graça AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title | AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title_full | AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title_fullStr | AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title_full_unstemmed | AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title_short | AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis |
title_sort | ap-1 and kif13a coordinate endosomal sorting and positioning during melanosome biogenesis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768840/ https://www.ncbi.nlm.nih.gov/pubmed/19841138 http://dx.doi.org/10.1083/jcb.200907122 |
work_keys_str_mv | AT delevoyecedric ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT hurbainilse ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT tenzadaniele ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT sibaritajeanbaptiste ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT uzangafsoustephanie ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT ohnohiroshi ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT geertswilliejc ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT verkleijariej ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT salamerojean ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT marksmichaels ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis AT raposograca ap1andkif13acoordinateendosomalsortingandpositioningduringmelanosomebiogenesis |