Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously....

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Detalles Bibliográficos
Autores principales: Chen, Bao-an, Lai, Bin-bin, Cheng, Jian, Xia, Guo-hua, Gao, Feng, Xu, Wen-lin, Ding, Jia-hua, Gao, Chong, Sun, Xin-chen, Xu, Cui-rong, Chen, Wen-ji, Chen, Ning-na, Liu, Li-jie, Li, Xiao-mao, Wang, Xue-mei
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2009
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775690/
https://www.ncbi.nlm.nih.gov/pubmed/19918366
Descripción
Sumario:Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe(3)O(4), and group D received DNR-loaded MNPs-Fe(3)O(4). For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe(3)O(4) can overcome MDR in vivo.

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