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Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously....

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Detalles Bibliográficos
Autores principales: Chen, Bao-an, Lai, Bin-bin, Cheng, Jian, Xia, Guo-hua, Gao, Feng, Xu, Wen-lin, Ding, Jia-hua, Gao, Chong, Sun, Xin-chen, Xu, Cui-rong, Chen, Wen-ji, Chen, Ning-na, Liu, Li-jie, Li, Xiao-mao, Wang, Xue-mei
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775690/
https://www.ncbi.nlm.nih.gov/pubmed/19918366
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author Chen, Bao-an
Lai, Bin-bin
Cheng, Jian
Xia, Guo-hua
Gao, Feng
Xu, Wen-lin
Ding, Jia-hua
Gao, Chong
Sun, Xin-chen
Xu, Cui-rong
Chen, Wen-ji
Chen, Ning-na
Liu, Li-jie
Li, Xiao-mao
Wang, Xue-mei
author_facet Chen, Bao-an
Lai, Bin-bin
Cheng, Jian
Xia, Guo-hua
Gao, Feng
Xu, Wen-lin
Ding, Jia-hua
Gao, Chong
Sun, Xin-chen
Xu, Cui-rong
Chen, Wen-ji
Chen, Ning-na
Liu, Li-jie
Li, Xiao-mao
Wang, Xue-mei
author_sort Chen, Bao-an
collection PubMed
description Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe(3)O(4), and group D received DNR-loaded MNPs-Fe(3)O(4). For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe(3)O(4) can overcome MDR in vivo.
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spelling pubmed-27756902009-11-16 Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo Chen, Bao-an Lai, Bin-bin Cheng, Jian Xia, Guo-hua Gao, Feng Xu, Wen-lin Ding, Jia-hua Gao, Chong Sun, Xin-chen Xu, Cui-rong Chen, Wen-ji Chen, Ning-na Liu, Li-jie Li, Xiao-mao Wang, Xue-mei Int J Nanomedicine Original Research Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe(3)O(4), and group D received DNR-loaded MNPs-Fe(3)O(4). For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe(3)O(4) can overcome MDR in vivo. Dove Medical Press 2009 2009-10-19 /pmc/articles/PMC2775690/ /pubmed/19918366 Text en © 2009 Chen et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Chen, Bao-an
Lai, Bin-bin
Cheng, Jian
Xia, Guo-hua
Gao, Feng
Xu, Wen-lin
Ding, Jia-hua
Gao, Chong
Sun, Xin-chen
Xu, Cui-rong
Chen, Wen-ji
Chen, Ning-na
Liu, Li-jie
Li, Xiao-mao
Wang, Xue-mei
Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_full Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_fullStr Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_full_unstemmed Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_short Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
title_sort daunorubicin-loaded magnetic nanoparticles of fe(3)o(4) overcome multidrug resistance and induce apoptosis of k562-n/vcr cells in vivo
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775690/
https://www.ncbi.nlm.nih.gov/pubmed/19918366
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