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Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo
Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously....
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775690/ https://www.ncbi.nlm.nih.gov/pubmed/19918366 |
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author | Chen, Bao-an Lai, Bin-bin Cheng, Jian Xia, Guo-hua Gao, Feng Xu, Wen-lin Ding, Jia-hua Gao, Chong Sun, Xin-chen Xu, Cui-rong Chen, Wen-ji Chen, Ning-na Liu, Li-jie Li, Xiao-mao Wang, Xue-mei |
author_facet | Chen, Bao-an Lai, Bin-bin Cheng, Jian Xia, Guo-hua Gao, Feng Xu, Wen-lin Ding, Jia-hua Gao, Chong Sun, Xin-chen Xu, Cui-rong Chen, Wen-ji Chen, Ning-na Liu, Li-jie Li, Xiao-mao Wang, Xue-mei |
author_sort | Chen, Bao-an |
collection | PubMed |
description | Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe(3)O(4), and group D received DNR-loaded MNPs-Fe(3)O(4). For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe(3)O(4) can overcome MDR in vivo. |
format | Text |
id | pubmed-2775690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27756902009-11-16 Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo Chen, Bao-an Lai, Bin-bin Cheng, Jian Xia, Guo-hua Gao, Feng Xu, Wen-lin Ding, Jia-hua Gao, Chong Sun, Xin-chen Xu, Cui-rong Chen, Wen-ji Chen, Ning-na Liu, Li-jie Li, Xiao-mao Wang, Xue-mei Int J Nanomedicine Original Research Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe(3)O(4), and group D received DNR-loaded MNPs-Fe(3)O(4). For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe(3)O(4) can overcome MDR in vivo. Dove Medical Press 2009 2009-10-19 /pmc/articles/PMC2775690/ /pubmed/19918366 Text en © 2009 Chen et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Chen, Bao-an Lai, Bin-bin Cheng, Jian Xia, Guo-hua Gao, Feng Xu, Wen-lin Ding, Jia-hua Gao, Chong Sun, Xin-chen Xu, Cui-rong Chen, Wen-ji Chen, Ning-na Liu, Li-jie Li, Xiao-mao Wang, Xue-mei Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title | Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title_full | Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title_fullStr | Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title_full_unstemmed | Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title_short | Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo |
title_sort | daunorubicin-loaded magnetic nanoparticles of fe(3)o(4) overcome multidrug resistance and induce apoptosis of k562-n/vcr cells in vivo |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775690/ https://www.ncbi.nlm.nih.gov/pubmed/19918366 |
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