Cargando…
Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae
BACKGROUND: Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776018/ https://www.ncbi.nlm.nih.gov/pubmed/19874630 http://dx.doi.org/10.1186/1471-2199-10-99 |