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Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

BACKGROUND: Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as...

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Detalles Bibliográficos
Autores principales:	Teste, Marie-Ange, Duquenne, Manon, François, Jean M, Parrou, Jean-Luc
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776018/ https://www.ncbi.nlm.nih.gov/pubmed/19874630 http://dx.doi.org/10.1186/1471-2199-10-99

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