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Comprehensive determination of (3)J(HNHα) for unfolded proteins using (13)C′-resolved spin-echo difference spectroscopy
An experiment is presented to determine (3)J(HNHα) coupling constants, with significant advantages for applications to unfolded proteins. The determination of coupling constants for the peptide chain using 1D (1)H, or 2D and 3D (1)H-(15)N correlation spectroscopy is often hampered by extensive reson...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777233/ https://www.ncbi.nlm.nih.gov/pubmed/19898942 http://dx.doi.org/10.1007/s10858-009-9382-3 |