In vivo expression and purification of aptamer-tagged small RNA regulators

Small non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators of bacterial gene expression. To study sRNAs and their potential protein interaction partners, it is desirable to purify sRNAs from cells in their native form. Here, we used RNA-based affinity chromatography to pu...

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Detalles Bibliográficos
Autores principales: Said, Nelly, Rieder, Renate, Hurwitz, Robert, Deckert, Jochen, Urlaub, Henning, Vogel, Jörg
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777422/
https://www.ncbi.nlm.nih.gov/pubmed/19726584
http://dx.doi.org/10.1093/nar/gkp719

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777422/
https://www.ncbi.nlm.nih.gov/pubmed/19726584
http://dx.doi.org/10.1093/nar/gkp719

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